![]() ![]() Suboptimal quality and/or quantity of transforming DNA Include a positive control in the transformation to verify competence and transformation efficiency of the prepared cells.(Also refer to the selection guide for competent cells) Make sure the properties of the selected cells (e.g., transformation efficiency, genotype) are appropriate for the transforming DNA and intended applications, such as methylated and unmethylated DNA, unstable constructs, large plasmids, ssDNA, library construction, etc.Consider electroporation over heat shock, for better efficiency with challenging DNA (e.g., library construction).Follow the transformation protocol and parameters (heat shock or electroporation) recommended for the selected competent cells. ![]()
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