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![]() ![]() However, Origin Sonic mistakes Eggman for a burglar attempting to rob the place, and tries to reach his office each night so he can kill him. Eggman applied for the nightshift at the location. Origin Sonic was fitted with a hat and bowtie and became the mascot of the restaurant.įor unknown reasons, Dr. After the cloning process, the machine produced a clone of Sonic with a different color scheme due to it being an early model. Tails decided to create a clone of Sonic with his permission, and created the cloning machine. ![]() However, due to being away most of the time, he wasn't able to attend everyday. ![]() ![]() After the events of the first Sonic the Hedgehog game, Sonic opened up a restaurant called Sonic's Fast Food Diner, a place where his fans and other children could meet him. ![]() ![]()
![]() ![]() Suboptimal quality and/or quantity of transforming DNA Include a positive control in the transformation to verify competence and transformation efficiency of the prepared cells.(Also refer to the selection guide for competent cells) Make sure the properties of the selected cells (e.g., transformation efficiency, genotype) are appropriate for the transforming DNA and intended applications, such as methylated and unmethylated DNA, unstable constructs, large plasmids, ssDNA, library construction, etc.Consider electroporation over heat shock, for better efficiency with challenging DNA (e.g., library construction).Follow the transformation protocol and parameters (heat shock or electroporation) recommended for the selected competent cells. ![]()
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